FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2760849
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12350.046.0561568
GTCTCGC13250.038.9411471
GTCTTGC25000.036.6266171
CTGTCGC16800.034.7138869
TCGCTCT14100.033.189314
TACTCCG1601.983968E-631.4979295
GTATTAA8600.030.4207781
TCTCGCT16500.029.2492732
CGCTCTG15100.029.084185
CTCGCTC16300.028.7097683
GTATTAG8950.026.795211
CGTTTCG1358.420431E-426.666369
GTACTAG6000.026.6463471
GTCTCAC31250.026.5106951
GTCTTAC10750.025.6886581
TTGCTCT34500.025.6678754
TAATACT8300.025.1549724
GTATTAT9450.024.608461
CTTGCTC34250.024.3837973
GTATACG1801.4969883E-424.2239531
Produced by FastQC (version 0.11.5)