FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1714094
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGATATATCTCGTATGCCGT41860.24421064422371236No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAT1001.3835198E-436.2870521
TCTGTCG5800.031.030098
TCGCTCT9050.028.685364
GTCTCGC8800.027.2152881
TCTCGCT9050.027.0917282
GTATAGG4550.025.5205651
CTCGCTC10200.024.744253
CGCTCTG9150.024.3935475
CCTTACG1500.001557452123.9966018
GTCTTGC14900.023.8666521
GTGTTAG9300.023.4110031
CTGTCGC10800.023.3300299
GTATTCT5400.021.5034371
TAGACGT1700.003191520521.2093564
TACCCGT1700.003220289721.1765615
TCCGTCG1700.003223014821.1734738
TAATACT4501.4188117E-1020.83234
GTATTAG4551.5097612E-1020.7354581
GTATTGA4952.1827873E-1120.526011
GGTTAAT5353.6379788E-1220.3478811
Produced by FastQC (version 0.11.5)