FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2804898
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12550.036.993921
TCTGTCG11750.035.5351378
TCGCTCT14350.033.1608924
CTGTCGC14100.031.6548659
CGCTCTG14600.029.5878055
GTGTTAA9700.028.4188141
GTCTTGC21200.028.4011751
TCTCGCT15300.026.8607182
CTCGCTC16000.026.1361833
GTGTACG3251.4788384E-924.5529331
GTATTAG6600.024.1809221
GTATAAG7550.024.020781
GTCTCAC27650.021.7759481
GTTCTAC6350.021.7057081
GTCGCTC3004.810536E-721.6299463
CTTGCTC28600.020.9240743
TTATACT6600.020.756014
TACACCG2455.4000942E-520.5705665
CGTTTCG1750.003813373220.5683679
GTGTTAG14500.020.0117951
Produced by FastQC (version 0.11.5)