FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2804898
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13500.034.9797441
GTCTTGC22300.032.2527961
TCGCTCT12500.031.6799964
TCTGTCG12450.031.2300248
CTCGCTC14950.027.4515023
CGCTCTG13300.026.526315
TCTCGCT16500.025.7518792
TTGCTCT29550.023.8781684
CTGTCGC17450.023.5194599
GGTCGAA2202.1952339E-523.1159861
GTGTAAG10700.023.0851231
GTATTAG7700.022.644231
GTGTTAG14650.022.3157731
TCTGTCA29750.022.0243118
GTGTTAT7700.021.7007221
GTCTCAC27450.021.4377771
ACCGTGT7250.020.8559138
GTACAGA11150.020.8502921
TTCACCG8450.020.4497015
TCTTGCT31400.020.4127372
Produced by FastQC (version 0.11.5)