FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2910793
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12250.038.7857448
CTGTCGC13500.035.1944739
GTCTTAC11150.029.9333271
GTCTCGC11950.029.7509061
TCGCTCT13700.027.368844
GTGTTAC10700.026.4455661
GTCTTGC25950.025.4434551
GTATAAT7800.025.1154651
CGCTCTG16000.023.8515835
GTGTAAA16400.023.0054931
TCTCGCT15750.022.890912
GTATTAT9750.022.3248561
CTCGCTC15400.022.0064873
TTACACT13250.021.767964
GTCCTAC8800.021.4369371
GTCTAGC11600.021.266351
GTATTGA9150.020.6169411
TAGACGT2806.390934E-620.6018184
GGGTTTC50650.020.4846531
GTGTAGG15300.020.3914931
Produced by FastQC (version 0.11.5)