FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2009717
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGCG750.001536514438.3926289
TCTGTCG7000.034.9638448
GTCTCGC8300.033.2062031
CTGTCGC8800.030.2669189
GTCTTGC16800.028.0619471
GTGTAAC6000.027.8029141
TCGCTCT8900.027.538024
GTGTAGG8800.026.3743081
TCTCGCT10700.024.9265252
CGTCAGA3551.546141E-1024.5169621
GTATTGG6150.023.5867771
CTCGCTC10100.023.5524653
GTGTAAG8800.022.253321
GTATTAG5550.022.2161961
CGCTCTG10300.021.6705955
GTATTAC3406.4121195E-821.332161
GTGTTAG13150.020.959051
TTCACCG6600.020.7279365
GTCTTAG6400.020.3988781
GTCTCAC20100.020.207181
Produced by FastQC (version 0.11.5)