FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2009717
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8950.042.229251
TCTGTCG8400.040.285368
TCGCTCT10000.036.7160224
CGCTCTG10200.034.5844885
CTCGCTC10250.034.4157833
GTCTTGC16550.032.498761
TCTCGCT11700.030.7804492
CTGTCGC11600.030.4135279
GTATTAC4600.028.4411891
TCTAGCG1358.401162E-426.676392
GTACTAG3652.1282176E-1023.8957961
GTGTAAA10200.023.5151021
TCTGTCA22400.023.4640798
TCGAACT13700.023.1238829
GGTATAC3803.5288394E-1022.952541
GTCGTGC1600.002144247322.713451
GTGTTAC5800.022.5568071
CTATACT5450.022.4562824
CTCGAAC14850.021.817998
GTTATGC4057.8216544E-1021.5357151
Produced by FastQC (version 0.11.5)