FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1449340
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG5800.037.235088
GTATTAA4100.035.934221
TCGCTCT7500.032.6344764
GTCTCGC7500.032.4126661
CGAATAC950.004894800530.3106653
GTCGTAA1000.005619973429.466061
CTCGCTC8200.028.9707073
TCTCGCT8500.027.9723382
CTGTCGC7650.027.2895019
CGCTAAG1409.108419E-426.308981
GTATTGA5050.026.2568861
GTCTTAC5650.026.076161
CGCTCTG8450.025.5578065
GTGTAAA8650.025.5486071
GTGCAAC5250.025.2566241
GGCGTAG1751.1271888E-425.2566221
GTCTTGC13350.024.830951
GTGTAAT6900.024.555051
GTTTCAC21500.023.7727223
GTATTAC3752.5283953E-1023.5728471
Produced by FastQC (version 0.11.5)