FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2334302
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9050.042.9536068
GTCTCGC8350.037.997391
CTGTCGC11250.033.274029
TCGCTCT11150.032.9268154
TCTCGCT11150.031.6593042
CTCGCTC11250.030.7144813
CGCTCTG11850.030.3742895
GTCTTGC22000.027.8373071
ATTACGC1050.00798232827.4236433
GTATAAC4750.024.8541091
TTGCTCT30150.024.5925964
GTATTAT8200.023.3954451
GTACTAC3502.8630893E-923.189771
CTTGCTC28600.023.1566773
GTGTTAG16000.023.058011
GTCTCAC26400.022.6387731
GTATTAA8350.022.0915071
GTATTAG8800.021.80031
GCGTATA2103.8335522E-421.081611
GCGTAGA2103.8335522E-421.081611
Produced by FastQC (version 0.11.5)