FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2334302
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10050.040.8969081
TCTGTCG9650.038.049968
TCGCTCT11550.036.1533134
TCTCGCT12400.033.6866072
CTCGCTC13550.031.3483583
ACGATCG950.00489252230.3143169
CGCTCTG13650.029.0089575
CTGTCGC12950.028.9097929
GTCTTGC19650.028.6229061
GTCGTAC1050.00791737527.4696031
TAACGCA1607.1649236E-526.998114
GTACAGG11600.026.7295821
GTATTAC6000.025.2376981
ACCGTGT7900.024.6063988
GGGTTTC37650.023.7486211
GTCTAAC5600.023.1774791
GTACTAT5600.023.1774791
GTCTCAC25850.023.1525731
GTACTAC4403.6379788E-1222.9433631
GTACTAA5400.022.7005751
Produced by FastQC (version 0.11.5)