FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1605576
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6150.046.8211678
GTCTTGC16000.037.779541
CGTTTCG800.002109147435.9937749
GGTATAC3050.031.4200131
GTATTAG6900.030.9821621
CTGTCGC9400.030.6339
GTCTCGC6750.029.4864711
GTACTAC3750.029.4864711
ATACCGG1050.00798133727.4238266
GTCTTAC6050.026.8058851
GTGTAGC7050.026.140491
GGTTAAC3707.2759576E-1225.9002821
GTACTAA3759.094947E-1225.5549411
GTCTCAC19300.025.2086431
TCGCTCT8150.024.7319184
GGGTTTC25950.024.7140961
CTATGCG1500.001557694223.9958489
TACCCGT1500.001557694223.9958485
CTACGCA1500.001557694223.9958489
CTGTTGC17150.023.5061369
Produced by FastQC (version 0.11.5)