FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1409516
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6450.055.8032268
GTCTCGC6500.051.185671
TCGCTCT6700.047.2744984
TCTCGCT7100.044.647572
CTGTCGC8900.042.0593349
CTCGCTC7450.040.5828063
GGTATAC3100.038.1599271
CGCTCTG8650.037.449455
GTAGTCG1301.4474319E-534.123781
GTCTTGC12800.030.613651
GTATAAT3650.030.3841881
GTCCGGT1100.008791408526.88541
CAGCGTA1358.427265E-426.6615439
TTCACCG4100.026.3364035
TAGTCGT1100.00997432926.1981622
TCGCACT1751.3182222E-424.6809714
GTATTGC3301.4133548E-924.6449531
GTATTAA3351.6825652E-924.2771171
TTGCTCT17250.024.2040424
CTGTTGC16550.023.9228949
Produced by FastQC (version 0.11.5)