FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1674268
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7450.044.4486858
GTCTTGC14450.038.7874871
GTCTCGC6850.037.6810841
CTGTCGC8800.037.6298529
TCGCTCT8150.033.5647474
TCTCGCT8800.031.9235952
CCGCGTT1306.759369E-427.687529
GTGTAAC4800.027.6552221
CGCTCTG9950.027.4927315
CTCGCTC9450.027.4238283
GTGTAAA9250.027.1071011
GTATAAA6200.026.1683851
TTGCTCT22350.026.0894474
ACCGTGT4700.026.0380488
GGTATAC3707.2759576E-1225.9112011
GTACTAG3707.2759576E-1225.9112011
TTACACT8200.025.4590074
GTCTAGA6800.024.9439281
GTATTAC3851.2732926E-1124.9016721
CCGTGTT5600.024.4243479
Produced by FastQC (version 0.11.5)