FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1674268
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC14500.038.311661
GTCTCGC8750.037.9278341
TCGCTCT8550.037.0498124
TCTGTCG7100.035.4934358
GTACGGG1751.05432264E-732.9807241
TCTCGCT9550.030.9151062
GCGCTAA950.00484296830.3769841
CGCTCTG9600.029.9977175
CTCGCTC10000.029.5177523
GTACTGA4700.029.1651361
GTTAACG1255.370773E-428.7978063
TATACTG5450.026.4200085
CTGTCGC10000.025.9203479
CTTGCTC19150.024.436783
TTGCTCT18100.024.2633464
GTATAGG3952.3646862E-1123.7440361
GTGTTAG11550.023.7361281
GTTTCAC23500.023.5896953
GTCTCAC18850.023.346771
GTACCCG1550.001861341523.272691
Produced by FastQC (version 0.11.5)