FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1608754
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7500.043.1974148
GTCTCGC5950.042.438781
TCGCTCT6750.037.33114
CTCGCTC6900.035.4761433
CTGTCGC9550.032.4169129
TCTCGCT8150.030.9251252
GTCTTGC14350.030.6683041
GTATAAA6650.029.2923321
CGCTCTG8200.028.9738775
TACCCGT1504.614862E-528.7982795
GATCCGT1255.3701666E-428.7982796
AATACGC1050.00797693527.426935
TAGTCCG1050.00797693527.426935
GTATAAG4300.026.8449971
CGTCAGG2559.9544195E-825.4632681
GTCCTAC4850.025.2882631
TAGGCCG2351.4147136E-624.5091745
GTGTAAA8850.024.4562451
TATACTG3652.401066E-1023.6698175
GTACTAA3402.6539055E-923.341331
Produced by FastQC (version 0.11.5)