FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1821212
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6600.035.99398
GTCTCGC8300.032.8475651
GTCTTGC16250.032.648161
TCGCTCT7750.032.510624
GGTATAC2755.456968E-1232.153491
TATACCG1050.00798147827.4239225
CGCTCTG8750.027.1496835
TCTCGCT9350.026.1982382
CTGTCGC8550.026.1008389
GGGTTAC4800.024.5616931
GTATTAT5500.024.1151161
GGGTTTC30100.023.990491
GGAGTGC40650.023.9073895
GTACTGG9000.023.742971
TAGCACT10400.023.5344734
GAGTGCA44400.023.509536
GTTTCAC27900.023.479893
CTCGCTC9550.023.3677673
TAACACT8650.023.302414
GTACTAT3853.4924597E-1022.9667781
Produced by FastQC (version 0.11.5)