FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1821212
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC7850.046.8761371
TCTGTCG8900.044.4906858
TCGCTCT8600.037.6712884
CTGTCGC12100.035.6995129
CTCGCTC8950.035.3937033
GTCTTGC16550.034.0053981
TCTCGCT10450.031.703332
GTATACG950.00484073130.3799951
CGCTCTG9950.028.218765
GTTCTAC5200.026.3634091
ACTGTCG2508.3882696E-825.9178458
GTCTACG1400.001039141325.7220342
GTGTTAC6300.025.1961061
TTACACT9550.024.8775124
TTGCTCT23350.024.357724
GTCTTAC6400.023.6750341
GTACTAA3052.1434971E-823.6565531
GTTACCC6400.023.6230353
GTGTTAG11450.023.315651
GTTAGCC7850.022.928033
Produced by FastQC (version 0.11.5)