FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1631981
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC7150.035.1112751
TCGCTCT8700.031.4423524
TACACTA3800.030.3100835
GCGTTAG1254.632216E-429.534781
GTCTTGC14850.029.3358921
CTCGCTC10000.028.0747153
TCTCGCT9000.028.0161862
CGCTCTG9000.027.9947285
TCTGTCG7000.026.7378228
CTCGATC6750.025.5951798
TCTGTCA20600.025.5097588
GTTCCGC1450.001102780525.4610141
GTGTAAC5550.025.2775141
CTACGTT2001.1264221E-525.1952554
TCTCGAT6700.024.7117637
GTCGTGC1500.001343746224.6123161
GTGTATA9200.024.0772651
GTGTTAC8100.023.700751
GCTACGT1851.921742E-423.3469523
GTCTTAC6350.023.2557321
Produced by FastQC (version 0.11.5)