FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1631981
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8000.034.1987468
GTCTTGC14800.034.127051
GGTATAC3950.032.8804551
GTATTAA4050.030.2870031
GTCTCGC6600.029.517681
GTATAAT5450.027.8025861
CTGTCGC10700.027.5877789
TTGCTCT18650.026.2502464
GTCCTAT5200.024.9764981
TCGCTCT8800.024.5438064
GTCTAGC7100.024.3901961
GTATAGG4750.024.3046151
GTATAAC3601.9645086E-1024.0514431
TCTCGCT9000.024.007952
GTATTGC4251.8189894E-1223.7684841
GTTTCAC23500.023.5899033
GTACTAA3402.6502676E-923.3440481
GGGCCGT3402.7175702E-923.2932666
GTTAGCC9900.023.2711643
GTGTTAT6250.023.0893841
Produced by FastQC (version 0.11.5)