FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1547452
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGA700.001090579341.1646162
GTACTAC3100.033.2933431
GTATCCG1000.00559932329.4883881
GTCTCGC6800.029.2715631
GTATTAA5050.027.7366031
TACGGAT1050.00798161627.4235677
TCTGTCG7000.025.7095958
TAGTACG1400.001042007425.7095954
GTACTCG1500.001356078424.5736581
TCTCGCT8200.023.7198542
GTGTAAG8400.023.6960281
GTCTTGC14250.023.2803061
TCGCTCT7450.023.19044
GTATAGT5600.022.3795811
GAGTGCA36050.022.3648536
GTATTAT4950.022.3396871
GTATTAG6150.021.576871
GTATAAG4801.0913936E-1121.501951
GTGTTAT7900.021.4630681
GGAGTGC32750.021.32135
Produced by FastQC (version 0.11.5)