FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1547452
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG5650.043.3252268
GTCTCGC6000.038.4776151
TCGCTCT7350.036.241884
CTCGCTC7350.036.241883
CTGTCGC7850.033.0174269
TCTCGCT8150.029.1622542
CGCTCTG8600.028.4627485
GGTATAC2603.92356E-927.748281
GTGTAAC5550.027.2983071
GTGTTAC6400.027.0545731
TTCCGAA1701.0790852E-425.4195042
GTCTTGC13300.023.8676931
GTATTAC4303.6379788E-1223.4892431
GTGTTAG10850.023.272751
ATACTAT5100.022.5870866
GTATTAA4800.022.5454791
GTTAGCC8650.022.4721183
GTCTTAC6450.022.3707071
GTATTAG5550.022.0986291
TGTTAGC11100.022.0607722
Produced by FastQC (version 0.11.5)