FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856337
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACCG1251.3275892E-534.558169
TCGCTCT10750.031.477394
GTCTCGC8650.030.9243261
GTCTTGC16900.030.8006861
TCTGTCG9350.030.0304858
GTACTAT4050.028.5614051
CTCGCTC10750.027.4593
TATTCCG1607.163329E-526.9985625
GTACTAC3505.456968E-1226.8528211
GGCTAGA10800.024.0986861
GTACCGG1500.001556700123.998726
CGCTCTG11350.023.4701145
CTGTCGC12950.022.7941519
GTACTAG4154.5474735E-1122.6469571
TAATACT6700.022.5659624
GTCCTAT4505.456968E-1222.4921071
TCTCGCT11550.022.4439892
GTATAGA6450.022.4173831
CTTGCTC21700.022.2292293
GTATAAG6200.022.1552431
Produced by FastQC (version 0.11.5)