FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1433029
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG5850.040.6133658
GTCTCGC6250.039.3086971
TCGCTCT6900.036.5199284
CTCGCTC7700.031.7906323
GTCTTGC12800.031.0486321
CGCTCTG7400.030.1606645
CTTACGA1204.2280764E-429.998519
TCTCGCT7950.029.8873942
CTGTCGC7800.029.5369959
GTACTAA2701.7826096E-1029.4387021
GTATACG1000.00617510128.9034541
GTCTCAC16150.024.1607821
TAATACT4201.8189894E-1223.9988064
GTATTGA6050.023.8871521
GTGTAAA8750.023.1227631
GTCTAAG4455.456968E-1222.7330531
GGAGTGC29950.022.596545
ATAGTAC3355.9240847E-821.4914683
GTACTAC2704.577445E-621.4099641
GAGTGCA33850.021.056566
Produced by FastQC (version 0.11.5)