FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1836730
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC6450.040.817861
TCTGTCG6950.040.3968438
TCGCTCT7700.030.8525354
CTGTCGC9500.030.311269
TCTCGCT9150.028.3467862
GTATAAA7550.026.1531641
GTATAAG5400.025.7316271
CTCGCTC9600.025.4961913
TCTACGG1751.3107614E-424.70222
CGCTCTG9050.023.8638365
GTGTTAG13900.023.6758251
GTCTTGC16500.023.4908851
GTACACG2252.4809924E-522.7521761
CTATTAG4505.456968E-1222.7521761
GTGTAAA11000.022.6044351
GTACTAA3604.3910404E-922.3458881
TAATACT6150.022.2405824
GTGTAAT7050.021.7839981
GTCTTAC7150.021.4793261
CGCACAT2053.45916E-421.4044881
Produced by FastQC (version 0.11.5)