FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1836730
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8200.037.7538348
GTCTTGC16100.032.757261
GTCTCGC7500.028.8981861
GTATTAA6150.028.1933521
ACCGTCG1050.00797723127.4269288
CTGTCGC11150.027.1194529
GTACGCA1358.413707E-426.6694242
GAATAAC5000.026.0083681
CGGGTCG1400.00104133725.7127445
TCGCTCT8900.024.2682084
GTCTAGC8050.024.2313981
GTCGACC1500.001556753623.9985628
GTACTAT4353.6379788E-1223.2514151
GTCTTAG6650.022.8143581
CTACGCA1902.3003302E-422.7354819
GTCTAGT7000.022.7057171
GTGTAAA11000.022.3304161
GAGTGCA45150.022.3242456
GTGTTAG12300.022.3197361
GGAGTGC41000.022.1255045
Produced by FastQC (version 0.11.5)