FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1715016
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6800.034.9346128
CTGTCGC8750.032.08549
GTCTCGC6300.027.948991
GTCTTGC15600.027.2771381
TAGCGCT1907.565721E-626.5213344
ACCGTGT5250.024.6810768
GTATTAA5650.024.6717871
GTATACG1500.00139480724.4553661
GTGTAGT10200.023.7360921
GTGTTAC7200.023.4363941
TTAACAC7450.023.1902733
CCGTGTT5900.023.1820859
TCGCTCT7500.023.035674
GTTCTAA5750.022.966781
GTGTAAT8150.022.5049361
TCTCGCT8350.022.4404642
GTGTAAA10800.022.4174181
TTCACCG5200.022.1496835
GTGTATA9000.022.009831
TTGCTCT23000.021.5959434
Produced by FastQC (version 0.11.5)