FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1729665
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG4550.032.1013221
TCTGTCG7050.029.6127938
GGGTAGC5800.027.7012311
GTCTCGC7650.027.6847721
GTACTAG4000.025.560681
GTATAAA6350.025.301911
GTCTCAC20100.024.706841
TCTCGCT9100.024.544552
GTCTTGC16600.024.1968571
TCGCTCT8550.023.5755834
TTACACT7700.023.3732874
GTATAAC3452.728484E-923.2850911
CTGTCGC9300.022.4484069
CTCGCTC9950.022.4289593
GTTACCC5250.021.9397263
GTGTTAC6350.021.851651
CGCTCTG8600.021.7643345
GGACCGT2654.0214636E-621.7327466
CGTAGTG2003.237689E-421.6150382
GTATTAT5750.021.591631
Produced by FastQC (version 0.11.5)