FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1729665
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7800.033.230498
GTCTTGC14600.031.606151
CGTTTCG1153.294211E-431.3040819
GTGTAAC4700.029.147431
GCGCTAC1306.697225E-427.731361
TAATACT5800.026.0687454
TACCGTC1400.001040999425.7140717
CTGTCGC10900.025.1007069
GGTACCG1801.5940964E-423.9997985
ATACTAT5100.022.5880436
GTGTAAT6100.022.4578551
GTTCGGG2003.2221194E-421.630461
GTGTTAC7000.021.6304591
TCGCTCT8400.021.428394
TATACTT4751.2732926E-1121.2208735
GGGTACG1700.003194172221.2063331
GTGTAAA10900.021.1674231
GCTATAC3509.4090865E-820.5712573
GTCTCGC8150.020.3476721
TTGCTCT21950.020.3369584
Produced by FastQC (version 0.11.5)