FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1526403
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6050.036.8870478
GTCTTGC12500.035.024071
GTATTAA5200.032.2737851
TACTCCG1153.2967562E-431.299675
GTATTAC3950.029.556181
GTCTTAC5550.027.6090661
CTGTCGC7750.026.937919
TAATACT5100.026.8195234
GTCTCGC7650.026.706811
GTACTAC2207.082126E-726.5333861
GTGTAAA9100.024.0549931
TCTCGCT8650.023.3257962
CTTTGCG2502.425024E-623.0365569
GTCATAC3203.1655873E-822.802131
CTTGCTC17400.022.7552193
TTGCTCT18350.022.7540914
TCGCTCT8300.022.5508464
GGAGTGC34900.022.4837465
GAGTGCA38800.022.2647116
GTGTTAC5900.022.261061
Produced by FastQC (version 0.11.5)