FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1524399
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGC850.002837244733.8819273
GTCTTGC14600.032.123941
GTCTCGC7250.029.8573681
GTATAAC2502.577508E-928.8621231
TCTGTCG6200.027.8697058
TCGCTCT7700.027.1165434
GTGTTAG10650.027.1005881
GTCGTTC1358.4203575E-426.6654598
GTACTAT3451.1459633E-1025.0974981
GTATTAA4650.023.2759061
GTCCTAC5300.023.1441561
GTATTAC3453.1359377E-923.0060391
CTGTCGC9200.022.6946229
CGCTCTG9550.022.6175175
ATAATAC5250.021.9425813
GTACTAA3956.2573235E-1021.9205991
TCTCGCT9200.021.9185222
CTCGATC4951.8189894E-1221.8171928
GTCTCAC15400.021.5528831
GTGTAAA10400.021.5078321
Produced by FastQC (version 0.11.5)