FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1545189
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTAT750.001430234338.956141
GTCTCGC6650.037.3451731
TCTGTCG6600.035.9954768
TCGCTCT8850.030.911374
GTACGGT1203.8854717E-430.4344861
CTCGCTC8900.029.1199363
GTCTTGC13100.028.9940741
TCTCGCT8700.028.9796962
GTATTAT5800.028.9652331
CGCTCTG8500.028.7963855
CGCGCGT1050.007979427.4251256
GTCGACT1358.424361E-426.6633173
GTCCTAT3850.026.5610071
GTACTAA3605.456968E-1226.3765541
CTGTCGC9750.025.8429099
GTATTAG5350.023.209851
GTCTAGA6250.022.2051
GTATTAC4006.2937033E-1021.9128281
GTGTTAC6050.021.7317311
CTTGCTC18800.021.4441153
Produced by FastQC (version 0.11.5)