FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1545189
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC6400.034.939971
GGTCCGT1601.9824747E-631.4994496
TCGCTCT7900.030.9867974
ACCCGAT1255.368743E-428.7994947
GTCTTGC14450.026.9566971
TCTGTCG7100.026.3657368
TAATACG1400.001041004725.7138354
TACCCCG1400.001041004725.7138355
TACACCG1400.001041004725.7138355
CTCGCTC8000.025.1995583
GTGTAAC4900.025.0261271
GTATTAC3251.5770638E-924.4146271
GTGTTAG11400.024.0447081
GTATAGA6000.024.0447081
TACACGG1801.5940871E-423.999585
GTGCTAA5450.023.8241161
TATACTG4303.6379788E-1223.441455
TCTCGCT8300.023.424312
CGCTCTG8350.022.418775
GTCTCAC17900.022.1641161
Produced by FastQC (version 0.11.5)