FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1514702
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6650.036.8111958
ACCGCGT1102.5381002E-432.726538
GTCTTGC14150.032.6746561
TTACCCG1000.006285028528.7993474
GTCTTAC7100.028.4896551
GGGTACG1804.8344773E-628.0939641
CTGTCGC9750.028.0609029
GCGTATA1306.6212687E-427.7852381
GTACGAT1050.00784516227.5206181
CTACACG1856.141392E-627.2426244
GTGTTAG10850.025.9670351
TAACACT6800.025.4111884
GTCTCGC6400.024.8330551
GTCTCAC17800.024.756961
GTGTAAA9750.024.451011
GGTATAC3301.8499122E-924.080541
TCGCTCT6900.023.9994544
GTCCTAT3652.2919266E-1023.750671
GTATAAC3453.092282E-923.0335581
GTACAAT5850.022.2281911
Produced by FastQC (version 0.11.5)