FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1462002
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAC4850.038.672561
CGTTTCG951.0722631E-437.8926859
GGTATAC2258.367351E-1032.0618671
GTCTTGC12950.030.638271
GTCTCGC6200.029.0883871
ATACCCG1050.00797515127.4280224
GTGTTAG10100.026.4272331
CTATACT4200.025.7137724
GTTAGCC8250.025.3085843
GTCCTAG7150.025.2234971
GTATTAA4151.8189894E-1224.3361171
TCGCTCT7200.023.999524
TCTGTCG6750.023.4653958
GTCTCAC16400.022.433531
GTATTAG5800.022.3880271
CTTGCTC16200.022.2217793
GTACTAC1952.7053244E-422.1966761
GTCCGGA1952.7053244E-422.1966761
TTGCTCT18850.022.1534044
GTGCTAA6600.021.8603631
Produced by FastQC (version 0.11.5)