FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2908942
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12100.039.2657368
GTCTCGC13400.034.894761
TCGCTCT13750.030.8895784
TACGTAC1204.2318896E-429.9951783
GTCTTAC10700.029.3609281
CTGTCGC16950.028.0304079
CTCGCTC15500.026.4731643
GTCTTGC26050.026.0831661
TCTCGCT16650.025.077482
GTATTAT6200.023.5680351
CGCTCTG16400.023.2645515
GTATAAG9000.021.9182721
GTTAGCC14400.020.9966243
TTGCTCT33650.020.9654254
GTCTCAC31400.020.9410251
GTGTAAC8400.020.8745461
TTACACT12550.020.6500664
GTACTAC3651.2560486E-720.0166871
TAACACT11900.019.9631774
TTACTCT15600.019.8429624
Produced by FastQC (version 0.11.5)