FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2908942
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC25500.031.7054181
TCTGTCG11200.030.8492478
GTCTCGC12200.027.3589761
TCGCTCT12800.025.3064584
CGTTTCG1851.9231258E-423.3453759
GTCTTAC9450.021.9772531
GTACTAC4407.2759576E-1121.9148161
ACTCGCG2003.2593237E-421.5944738
CTCGCTC13800.021.3861963
CCGTTAG1750.003206908721.192351
GGTATAC5650.021.0048081
TCTCGCT15350.020.6345712
GTGTAAA15950.020.461581
GTGTTAG17000.020.0704021
GATCGTT3601.2706005E-719.994889
GTGCTAA11650.019.7370821
GTATTAA6800.019.6340891
GTCCTAA9500.019.519271
CTGTCGC19150.019.1700279
CGCTCTG14800.018.9684435
Produced by FastQC (version 0.11.5)