FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2864061
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10650.030.4175388
GTATTAA8750.029.2279741
GTCTTGC23250.028.2851351
GTCTCGC12500.028.0588551
CGACCGG1607.1675255E-526.9969795
TCGCTCT13650.025.8432624
CTGTCGC13650.024.259759
GTGTAGA13750.022.8509621
TTATACT8550.022.7342974
CGCTCTG13700.022.5960125
CTCGCTC14250.022.229093
GTGTAAC7250.022.1729451
GTATTAT8750.021.712211
GAGTGCA66500.021.1104186
TCTCGCT14550.020.781182
GTCTTAC10600.020.680171
GGAGTGC61850.020.3695855
TATACTG7250.019.8598465
GTATAAT8850.019.8155751
ATACTTT12150.019.5533686
Produced by FastQC (version 0.11.5)