FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2864061
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13250.032.5950248
GTCTCGC12400.032.2988241
TCGCTCT13500.031.991414
TCGCGCA1050.00798608427.421214
GTCTTGC24850.025.6676881
CGCTCTG14100.025.0145595
CTCGCTC14250.024.751253
GTCTTAC10550.023.1993681
TCTCGCT15250.023.1310442
CTGTCGC19050.023.048939
CCGGACT2654.0273717E-621.7300154
GTACAGA15200.020.49371
GTATAGA10900.020.413121
GTGTTAG17950.020.246331
TTGCTCT34400.019.8783864
GTATTGA9500.019.5178091
GTGTAAC8800.019.3847331
TTATACT9750.019.1948454
GTCTAGT13150.019.1764321
ATAATAC7950.019.0137633
Produced by FastQC (version 0.11.5)