FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2583162
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10500.034.044891
TCTGTCG9950.032.5590328
TCGCTCT12400.026.7065734
GTGTTAC10100.026.003171
TCTCGCT13250.025.5356672
GGTATAC5450.024.0946791
GTCTTGC23500.023.9038581
GTATAAC5200.023.8501281
CTCGCTC13100.023.081293
GTATTCG1952.5082324E-422.4471781
TATACTG7700.022.4393845
CTGTCGC15300.022.1150769
GTCTCAC27550.021.7138791
GTCTTAC9500.021.5020351
CTGTTGC26350.021.3106179
CGCTCTG12850.021.2897685
GTGTTAG14300.020.4065271
TCTGTTG26900.020.3396458
GTATTAT7600.020.1581571
GTACTAG4752.5102054E-1019.9661751
Produced by FastQC (version 0.11.5)