FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2583162
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9450.039.1794661
TCTGTCG11400.039.1474768
TCGCTCT11400.035.359694
TCTCGCT12800.029.8080312
GTCTTGC21800.029.2120691
CTCGCTC12650.029.0205083
CTGTCGC16600.027.3180319
CGCTCTG13500.026.1273925
TACACCG1701.0832664E-425.405995
TTGCTCT31700.022.7073334
GTATAAG6900.022.536711
GTCTCAC27950.022.25451
GTGTAAG11950.021.6880471
GTCTAAC5151.8189894E-1221.5677281
GTATTAG9100.021.1569141
GTATAAA10250.020.9505041
GTACTAT6150.020.4688821
GTTCTAA8700.020.4273641
GTCTAAG8850.020.0811391
GTGTTAC9600.020.0549891
Produced by FastQC (version 0.11.5)