FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2620984
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11300.041.4098058
GTCTCGC11250.035.743041
TCGCTCT11900.035.69224
GTCTTAC9650.034.8507271
CTGTCGC15850.031.7934049
GTCTTGC22100.031.4277041
TCTCGCT13850.028.5878312
CGCTCTG13250.027.7096375
CTCGCTC14250.025.2594243
TTGCTCT32600.024.070064
CCGTACC3301.9281288E-923.996454
GTATTAG8400.023.4998871
TTACACT11900.022.9881974
GTGTTAC9250.022.1308271
CTTGCTC31150.021.492813
GTGTAAA14700.021.3861431
GGGTTTC40750.021.1707251
GTATTGA7300.021.0318661
GTCTCAC27350.020.5832861
TAACACT11100.020.1051354
Produced by FastQC (version 0.11.5)