FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3285939
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12950.033.9104088
GTCTCGC14000.030.8065411
GTCTTGC28900.027.823631
TCGCTCT16150.025.8548264
CGTTTCG2301.1744578E-625.0400459
CTGTCGC16550.024.7941829
GTATTAT9050.024.2321341
TAATACT8200.023.7047884
GTGTAAA18850.023.2679881
TCTCGCT17600.022.9066452
GTATAGG9900.022.889981
CGCTCTG16500.022.6884865
GTATTAA8400.022.6262721
GTATAAG10100.022.4367161
GTGTTAT12600.022.0461121
TACCCGT2303.320118E-521.9107065
CTCGCTC18400.021.9107063
TTGCTCT39850.021.4983334
GTATAGA9650.021.2104381
GTCTCAC32850.020.917581
Produced by FastQC (version 0.11.5)