FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3063961
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12200.037.174428
GTCTCGC12050.036.988611
TCGCTCT14350.031.6057624
TCTCGCT14050.030.7434482
CTCGCTC14100.030.634433
CTGTCGC16350.027.7387149
GTCTTGC27900.027.498581
GTATTAG10400.027.4003731
CGCTCTG15800.027.3383225
GTATTAC6850.026.667031
GTACGGA2756.2354957E-926.5700591
GTCTCAC32700.025.0262361
GGGTTTC48200.024.8611971
GTATTAA8750.024.216711
GTATTAT7900.024.0475851
GTTTCAC45050.023.9703373
GTGTAAG16900.023.3470651
GTACTAT6950.023.1293321
TTCACCG8750.023.0370925
GGTTACG2002.9455664E-421.92031
Produced by FastQC (version 0.11.5)