FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3063961
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14600.034.513128
GTCTCGC13000.031.9348351
GTCTTGC27250.031.83021
GTATTAC6300.029.4184281
GTACTAA6900.026.8603041
TCGCTCT14950.026.4830284
TCTCGCT16300.026.0595722
TTATACT9650.023.8709034
GTATTAG10350.023.6370681
GTATAGG8800.023.588231
GTGTTAG20450.023.5635151
CTGTCGC22000.023.55789
CTCGCTC16050.023.3224643
CGCTCTG16600.022.9833815
GTGTTAT12750.022.6764181
GGTATAC5600.022.5050981
TTGCTCT38150.022.076874
GTCTCAC35700.021.8042471
GGGTTTC48200.021.6866321
CGGTACT2003.2581194E-421.5957094
Produced by FastQC (version 0.11.5)