FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2913419
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12050.038.833288
GTCTCGC12450.032.883751
CTGTCGC16100.029.5118059
GTATAAT7650.027.7140031
TCGCTCT13150.027.3725034
GTCTTGC26300.026.685671
GTATTAG9600.025.8922841
TCTCGCT14600.025.146652
CGCTCTG15200.024.6284755
GTCTAAC6850.024.5470871
CTCGCTC14400.024.496493
GTATAAG9850.024.492911
GCGTACA1851.731071E-423.710581
GTATTAC7800.023.431931
GTATTAT9000.022.7445931
TATACTG9200.022.692795
GTTACCG2303.3208875E-521.9099043
GTCGTAG2353.4808145E-521.776741
GTACTAT7700.020.8878921
GTGTAAA19750.020.7292481
Produced by FastQC (version 0.11.5)