FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2913419
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10900.037.383131
TCTGTCG11150.035.506938
GTCTTGC27500.034.483931
TCGCTCT14000.033.420894
GTCGTAC1305.714609E-428.4948331
GTATTAG10850.027.9958421
CTCGCTC15700.026.5926173
TCTCGCT16700.026.2951662
CGCTCTG14450.025.9045265
GTACTAC6800.023.9691831
CTGTCGC17100.023.5731269
GGTATAC6600.023.5729981
GTACTAA6600.023.5729981
TTGCTCT36450.023.303294
CTTGCTC37100.022.3129333
TCTGTCA38000.021.4052418
GTCTTAC11450.021.3524591
GGCGTAC2103.729935E-421.167591
GTATTAC7050.021.0174661
GTGTAAT11350.020.8878421
Produced by FastQC (version 0.11.5)