FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2616308
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC23150.034.693411
TCTGTCG11650.031.5104548
GTCTCGC11200.029.3359281
CGGATAC1050.00798719827.4203363
TCGCTCT12200.027.1393934
GTGTAAT9600.026.6196371
GTGTTAC9350.026.5504951
GTACTAT4850.025.592491
GTGTAAC8100.025.239361
CGCTCTG11600.024.8201315
GTATTAC6000.024.3379551
CTCGCTC13950.024.2507823
TTGCTCT31400.023.8399754
TCTCGCT15400.022.9100952
GTCCTAC8100.022.5351431
CTGTCGC17150.022.2431969
GTGTAAA13450.021.714161
GTCTCAC27000.021.6337381
GTATTAG9150.021.5450741
CTTGCTC31850.021.469223
Produced by FastQC (version 0.11.5)