FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2260713
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8550.041.2571758
GTCTCGC10100.035.8540951
GTCTTGC18150.032.721061
TCGCTCT10900.029.0709384
CTGTCGC12350.027.3968359
TCTCGCT12750.026.547312
CTCGCTC12100.025.592693
CGCTCTG12500.024.1959845
GTGTAAA13200.024.141761
TACGCAC1500.001554228624.0055473
GTATTAG8250.023.7028181
TTGCTCT26900.023.0239074
GTCCTAT5350.023.0136411
GTCTCAC23000.022.9871541
GTCTTAC9050.022.4078221
TCTGTCA26600.022.192278
GTATATA9400.020.8030051
GTATAGA6650.020.6929381
GTGTAAC6400.020.369611
GTATTAC5700.020.3299031
Produced by FastQC (version 0.11.5)