FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2728431
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC23950.033.5247881
GTCTCGC10100.031.798761
GTCGTAC950.004574636530.7337281
TCTGTCG10000.030.2304638
TCGCTCT12950.026.6788434
GTACCGT1500.001559264123.9924346
GTCCGGA3051.8964784E-823.9320011
TCTCGCT13050.023.7236122
GTGTTAG17250.023.696151
GTATTAG9400.022.5189931
TTACACT13850.022.346744
GGTTAAC5600.022.158471
TAATACT6850.022.066034
GTCTTAC9950.022.007821
GTCCTAG12300.021.9571231
GTATTAC6700.021.7888371
TACACTG15900.021.7289925
GTATAGC5051.8189894E-1221.6809711
CTTGCTC33600.021.636033
CTCGCTC13800.021.384563
Produced by FastQC (version 0.11.5)