FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005346902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005346902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2126215
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8400.028.6829991
GCGCTAT1306.2242494E-428.0812591
GTCTACG1606.5124994E-527.3792251
GTACTGA6000.025.5539461
TCGCTCT8900.024.2609464
GTATTAC3503.2341632E-922.9464021
TAACCCG2202.3594404E-522.9008625
GTATAGG5450.022.7741581
GTCTTGC18350.022.6792531
GTATTAA4204.7293724E-1122.5987261
TCTGTCG9300.022.4446078
TTAGACT6600.021.8103474
GTACTAG3355.111542E-821.7944091
GTGTTAG13000.021.3417571
TCGAACT12800.020.8045359
CTCGCTC10050.020.7686583
CGTATAT1750.00380992920.5712992
GAGTGCA46200.020.5640416
GTACAAA8550.020.4943941
ATACTTT7400.020.4250956
Produced by FastQC (version 0.11.5)